Factors afecting enzyme activity Essay Example for Free

Factors afecting enzyme activity Essay Below is a table of result which I obtained when conducting these experiments. Time (s) Amount of gas given off using 1cm3 of liver suspension and 1cm3 of hydrogen peroxide (cm3) 0. 0 Amount of gas given off using 2cm3 of liver suspension and 3cm3 of hydrogen peroxide(cm3)   Amount of gas given off using 1cm3 of liver suspension and 3cm3 of hydrogen peroxide(cm3) 0 Amount of gas given off using 1cm3 of liver suspension and 4cm3 of hydrogen peroxide(cm3). Amount of gas given off using 1cm3 of liver suspension and 5cm3 of hydrogen peroxide(cm3)   Amount of gas given off using 0. 5cm3 of liver suspension and 5cm3 of hydrogen peroxide(cm3) 0By looking at these results I can see that the best ratio I have tested so far is the ratio of 10:1 (5cm3 hydrogen peroxide to 0. 5 cm3 liver suspension) as it gives me a good spread of results and does not happen so quickly that I cannot take results from it. I obtained a 1cm3 syringe with which I could accurately measure to the nearest 0. 02 cm3 I used 0. 2cm3 of liver suspension and 5cm3 of hydrogen peroxide my results are in the table below. Time (s): 0. 0   Amount of gas given off using 0. 2cm3 of liver suspension and 1cm3 of hydrogen peroxide (cm3his ratio gave me good results which I can easily analyse so I have decided to use this ratio in my final experiment. I will use the concentrations 0%, 2%, 4%, 6%, 8% and 10% of liver suspension in my experiments as these should give me sensible results. Also, after conducting these experiments I have decided to use a 1cm3 syringe as this will give me accurate results and I have decided to use a gas cylinder rather than a measuring cylinder to collect gas as this is more accurate and easy to read. Fair testing In order to ensure that my results are reliable and accurate I will endeavour to make sure that there is only one variable in all of my experiments. Given that I only wish to test one variable I will make sure that I control all other variables that could affect the amount of gas produced in my experiments. Factors that could affect the results of my experiments are temperature, pH, apparatus and substances. To maintain a constant temperature throughout all my experiments I will conduct all of my experiments with the side arm boiling tube in an electronically heated water bath set at a temperature of 30i C. I have chosen this temperature as it is higher than room temperature so this will not affect it and it is not at a temperature high enough to denature the enzymes. To control pH I will add a pH buffer to the liver suspension buffering the pH at seven. I have chosen seven as it is neutral and therefore should not affect my experiments. I will also test the pH of the liver suspension at the start of each experiment using universal indicator if the paper turns light green I will know the pH is seven. To ensure that my apparatus is in working order I will ensure that I assemble the apparatus well and double check that all connections are well made and are therefore as air tight as possible. This will mean that any all gas produced goes into the gas cylinder and does not escape the apparatus so that my results are accurate. Given that all substance concentration that I will ask for will be mixed by the school biology department I cannot be sure exactly what is in them so unfortunately I will have to trust that all the substances I use are what they should be. Before using any of my substances I will stir them for ten seconds using a glass rod, this is to ensure that there is an even distribution of liver in my suspension so my results are accurate. Also, I will always use the same concentration of 10 vols hydrogen peroxide. Another factor which could affect my results is human error. When I am conducting my experiments it is possible that I could inaccurately measure my substances and I could start the stop clock at the wrong time. To accurately measure all of my substances I have chosen the most accurate apparatus to measure them with. I will measure as closely as is possible with the human eye measuring my liver suspension accurate to the nearest 0. 01cm3 and my hydrogen peroxide to the nearest 0. 1cm3. Also, when injecting my hydrogen peroxide I will inject it as quickly as possible so the full amount is in the boiling tube as soon as possible. I will start the stop clock at the very moment I inject the hydrogen peroxide so my results are accurate. Safety When conducting my experiments it is vital that I take the utmost care to be safe in the laboratory. I will wear safety glasses to protect my eyes as well as tucking my tie into my shirt to avoid it coming into contact with any apparatus or substances. Also, when dealing with hydrogen peroxide I will only remove the stopper from the bottle when I need to use it, I will extract it with a syringe and replace the stopper immediately. I will do this as hydrogen peroxide is highly dangerous and can cause the spontaneous combustion of clothing if applied in high concentrations. Also I will take great care when handling glass equipment and will ensure that all apparatus is properly clamped in place before beginning my experiments. I will be aware of others around me, tidying my apparatus away so it does not pose a danger to others. Also, I will wash my hands after conducting my experiments so as not to leave any dangerous substances on my hands which may be ingested if I put my hands near my mouth. MethodApparatus   A water bath   A trough A side arm boiling tube   A test tube rack   A gas cylinder   A stopwatch   A clamp and retort stand   1x 1cmi syringe   1x 5cmi syringe   A bung with a space for a syringe. Thermometer Diagram Procedure Before conducting any experiments I will ensure that the gas cylinder is full of water with no air bubbles by filling it then placing my thumb over it whilst submersing it in the trough. I will also ensure that the water bath is at a temperature of 30i C. After collecting the listed apparatus I will set it up as in the diagram above. I will then remove the bung and collect a liver suspension. I will begin with a concentration of 2% then proceed to use 4%, 6%, 8% and 10% as well as a control of distilled water. I will firstly take a sample of the solution in a test tube and universal indicator solution to it to in order to monitor the pH of the suspension. I will not add a buffer to control the pH as the chemicals in the buffer could interfere with the reaction and alter my results. I will measure out 0. 2cm3 of the suspension using a 1 cm3 syringe being as accurate as is possible with the naked eye when measuring. I will inject this suspension into the side arm boiling tube and replace the bung. I will then use the 5cm3 syringe to measure out 5cm3 of hydrogen peroxide. I will place the syringe into the bung and ensure that all my apparatus is air tight. I will then quickly inject all of the hydrogen peroxide into the boiling tube whilst simultaneously starting the stop clock. I will then record the amount of gas collected in the gas cylinder at ten second intervals for a period of two minutes. I will then repeat each experiment three times for each concentration of liver. I will record all my results on the table below. After collecting my results I will go on to test a different liver concentration until I have three sets of data for five different concentrations as well as a control. I will then tabulate these results and use the average results for each experiment to plot a graph of my results allowing me to analyse them easily. Taking the average of three experiments for each suspension will give me more reliable results as it will reduce the affect of anomalous results. Also, to ensure that all my tests are fair I will endeavour to use exactly the same conditions for each experiment by maintaining a constant temperature and by measuring all substances as accurately as is possible. I will also ensure that my apparatus is set up in exactly the same way for each experiment. When conducting my experiments I will consider all factors which I discussed in the fair testing section of this project making my results as reliable and accurate as possible. Observations On the next page is a table showing my results for the experiments described in my plan. I will go on to analyse these results in the analysis section. Analysis On the next page is a graph showing volume of gas produced against time for the six concentrations of liver suspension I used in my experiments. Although the graph of my results that I produced may at first appear not to agree with my predicted graph it does in fact indicate that my hypothesis was true. The lines on my graph are in the same positions as I predicted apart from the control graph which was higher than I expected. The lines did not flatten out as I predicted however, I believe this is due to the fact that I used a short time scale and the graphs would have flattened out had I used a longer time scale. I did not use a longer time scale as it would have been pointless given that I am investigating the rate of reaction of several different liver concentrations rather than the rate of reaction changing over time in one concentration of liver. On the whole I am happy with my graphs and I think they verify my hypothesis. My graph for the reaction rate of the 2% liver concentration followed this pattern well. Initially there was a dramatic increase in the amount of gas released. After the first ten seconds this slowed significantly, the amount of gas released still increased but it increased much more slowly and steadily. This was because the catalase broke down the hydrogen peroxide into water and oxygen slowly and steadily. The reason for the graph continuously going up slowly throughout the entire two minute period was because there was little catalase compared to hydrogen peroxide meaning that even by the end of the two minutes there was still a lot of hydrogen peroxide which had not been decomposed and so the enzymes were still working at the same rate. I think that if I had let the experiment continue for a longer time period the gradient of the graph would have flattened to zero as all of the hydrogen peroxide would have been decomposed. My line is approximately straight showing me that the reaction rate was roughly the same throughout the experiment however, the gradient is steepest at approximately sixty seconds meaning that the rate was highest here. In order to compare the rates of reaction of all my graphs I will need to calculate the rate of reaction for each graph. I will do this by dividing the amount of oxygen produced by the time taken to produce it. This will give me a rate in cmi of oxygen per second (cmi /s). I have chosen to take the rates of reaction from the points on the graphs after thirty seconds. Although it may seem that the maximum rate is at ten seconds and so I should take this rate I think this is due to the hydrogen peroxide I injected displacing air in the apparatus. I have chosen thirty seconds as I think this is sufficient time for the experiment to have settled after the initial burst of gas and it is not too late that some of the reactions had begun to slow down. The rate of reaction at thirty seconds for the 2% concentration suspension is as follows: 3. 83cmi 30s =0. 13cmi /s (2dp) I chose to take my rates to two decimal places as this is accurate enough for me to analyse my rate graph well and it is not so accurate that it would be difficult to plot on a graph. My graph for the rate of reaction of the 4% concentration liver suspension further supports my hypothesis. It has the same initial increase in gas from when I injected the hydrogen peroxide, it then continues to slope upwards but not as fast as before. As I predicted the 4% graph slopes up at a higher gradient than the 2% solution graph. This can be seen by simply looking at the graph. As predicted the gradient of each line is higher than the one that preceded it. This is because as the concentration of the suspension increases there is more catalase to break down the hydrogen peroxide into its component parts. I will now proceed to compare the gradients and rates of reaction for each concentration. On this graph the gradient of the line and the rate of reaction are the same thing as gradient=change in Y Change in X And rate of reaction=amount of gas produced (change in Y axis) Time (change in x axis) Below is a table showing the rates of reaction for each of my concentrations of catalase including the control experiment of 0% catalase. As before I will take the gradient of the line after thirty seconds. concentration of liver suspension amount of gas produced (cmi ) time (s) rate of reaction (cmi /s). On the next page is a graph of my results, I have plotted concentration of liver suspension against rate of reaction, this will better show my results and will help to verify my hypothesis. As you can see from the graph there is clearly a relationship of proportionality between the rate of reaction and the concentration of liver suspension as I predicted in my hypothesis. I have added a best-fit line to my graph to better show this trend. As I predicted the line is slopes upwards showing that as the concentration of liver suspension increases the rate of reaction increases, this is due to the fact that there was more catalase to collide with the hydrogen peroxide in the higher concentrations. One problem with these results is that my control experiment seems to have a rate of reaction however, this is simply due to the initial burst of gas at the start of the experiment when the hydrogen peroxide that I injected displaced air in my apparatus. Although this effectively means that all of my results are wrong I can still draw sensible conclusions from my graphs as every one of my results had the same displacement of air so when comparing my results this in fact has no effect. If there had been no displacement of air in my apparatus I think this line would have been straight and through the origin showing that rate of reaction and concentration of liver suspension are directly proportional. I will not attempt to subtract the gas displaced by the hydrogen peroxide from my results as this could further magnify any inaccuracies in my experiments and I do not need to in order to draw reliable conclusions from my graph. The conclusion I have come to by looking at my graphs is that my hypothesis was correct. I think that as the concentration of the liver suspension increases so does the rate of reaction proportionally.

Obedience and Submissiveness in Samuel Becketts Waiting for Godot Ess

Obedience and Submissiveness in Waiting for Godot Samuel Beckett's pessimistic attitude about the existence of man lead him to write one of the best contemporary plays known to the twentieth century. Even with its bland unchanging set, clown-like characters, and seemingly meaningless theme, Waiting for Godot, arouses the awareness of human tragedy through the characters' tragic flaws. Charles Lyons feels, a character's attitude of the space in which he lives, shows a range of detail marking economic status, social classification, and psychology (Lyons 19). Beckett uses the character, Lucky, as a metaphor for Man. Using physical, mental, and social blemishes, Lucky exemplifies Becketts idea that universal man is a slave to his own being. First Lucky symbolizes man's slavery in a physical sense. Lucky has a master that instructs him where to go and what to do. Lucky is physically tied with a rope to his master, but in a sense is also tied to him by fear of being alone. Lucky is asked by two tramps to dance, but refuses. Lucky only dances at his master command. Lucky is also a slave to weakness. When Lucky does finally dance, he shuffles chaotically. Ramona Cormier and Janis Pallister describe Lucky's movements as stiff and ungraceful. They believe it is because he is use to being loaded down with burdens...his body is unable to move freely (Cormier and Pallister 13). Brooks feels that age has diminished Lucky's dance to a few ineffectual, spasmodic memories of a past ritual (Brooks 294). Lucky calls his dance the net (Beckett, Act I 27). It is ironical that Lucky does not escape the net that restricts him from being independent. The last physical characteristic of slavery that Lucky exemplifi... ... C. "The Mythic Pattern in Waiting for Godot." Modern Drama 9 (1966/67) 292-299. Carey, Gary, and James Roberts, eds. Beckett's Waiting for Godot, Endgame, & Other Plays. Cliffs Notes, Inc. Nebraska: 1995. Cromier, Romona.,and Janis L. Pallister. Waiting for Death. Alabama: U of Alabama Press, 1979. Fletcher, J. "Action and Play in Beckett's Theater." Modern Drama 9 (1966/67) 242-246. Iser, W. "Beckett's Dramatic Language." Modern Drama 9 (1966/67) 251-259. Lamont, Rosette. "Beckett's Metaphysics of Choiceless Awareness." Samuel Beckett Now. Ed. Melvin J. Friedman. Chicago: U of Chicago Press, 1975. 199-217. Lyons, Charles R. Samuel Beckett. New York: Grove Press, 1983. Metman, Eva. "Reflections on Samuel Beckett's Plays." Samuel Beckett: A Collection of Critical Essays. Ed. Martin Esslin. New Jersey: Prentice-Hall, 1965

Software Developer

R N S INSTITUTE OF TECHNOLOGY CHANNASANDRA, BANGALORE – 61 UNIX SYSTEM PROGRAMMING NOTES FOR 6TH SEMESTER INFORMATION SCIENCE SUBJECT CODE: 06CS62 PREPARED BY RAJKUMAR Assistant Professor Department of Information Science DIVYA K 1RN09IS016 6th Semester Information Science and Engineering [email  protected] com Text Books: 1 Terrence Chan: Unix System Programming Using C++, Prentice Hall India, 1999. 2 W. Richard Stevens, Stephen A.Rago: Advanced Programming in the UNIX Environment, 2nd Edition, Pearson Education / PHI, 2005 Notes have been circulated on self risk nobody can be held responsible if anything is wrong or is improper information or insufficient information provided in it. Contents: UNIT 1, UNIT 2, UNIT 3, UNIT 4, UNIT 5, UNIT 6, UNIT 7 RNSIT UNIX SYSTEM PROGRAMMING NOTES UNIT 1 INTRODUCTION UNIX AND ANSI STANDARDS UNIX is a computer operating system originally developed in 1969 by a group of AT&T employees at Bell Labs, including Ken Thompson, Dennis Ritchie, Do uglas McElroy and Joe Ossanna.Today UNIX systems are split into various branches, developed over time by AT&T as well as various commercial vendors and non-profit organizations. The ANSI C Standard In 1989, American National Standard Institute (ANSI) proposed C programming language standard X3. 159-1989 to standardise the language constructs and libraries. This is termed as ANSI C standard. This attempt to unify the implementation of the C language supported on all computer system. The major differences between ANSI C and K&R C [Kernighan and Ritchie] are as follows: ? Function prototyping ? Support of the const and volatile data type qualifiers. Support wide characters and internationalization. ? Permit function pointers to be used without dereferencing. Function prototyping ANSI C adopts C++ function prototype technique where function definition and declaration include function names, arguments’ data types, and return value data types. This enables ANSI C compilers to check for function calls in user programs that pass invalid number of arguments or incompatible arguments’ data type. These fix a major weakness of K&R C compilers: invalid function calls in user programs often pass compilation but cause programs to crash when they are executed.Eg: unsigned long foo(char * fmt, double data) { /*body of foo*/ } unsigned long foo(char * fmt, double data); eg: int printf(const char* fmt,†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. ); External declaration of this function foo is specify variable number of arguments Support of the const and volatile data type qualifiers. ? The const keyword declares that some data cannot be changed. Eg: int printf(const char* fmt,†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. ); Declares a fmt argument that is of a const char * data type, meaning that the function printf cannot modify data in any character array that is passed as an actual argument value to fmt.Volatile keyword specifies that the values of some variables may change asynchronously, giving an hint to the compiler’s optimization algorithm not to remove any â€Å"redundant† statements that involve â€Å"volatile† objects. char get_io() { volatile char* io_port = 0x7777; char ch = *io_port; /*read first byte of data*/ ch = *io_port; /*read second byte of data*/ } ? eg: If io_port variable is not declared to be volatile when the program is compiled, the compiler may eliminate second ch = *io_port statement, as it is considered redundant with respect to the previous statement. Prepared By: RAJKUMAR [Asst. Prof. ] & DIVYA K [1RN09IS016] Page 1 RNSIT UNIX SYSTEM PROGRAMMING NOTES The const and volatile data type qualifiers are also supported in C++. Support wide characters and internationalisation ? ? ANSI C supports internationalisation by allowing C-program to use wide characters. Wide characters use more than one byte of storage per character. ANSI C defines the setlocale function, which allows users to specify the format of date, monetary and real number re presentations. For eg: most countries display the date in dd/mm/yyyy format whereas US displays it in mm/dd/yyyy format. Function prototype of setlocale function is: ? #include char setlocale (int category, const char* locale); ? The setlocale function prototype and possible values of the category argument are declared in the header. The category values specify what format class(es) is to be changed. Some of the possible values of the category argument are: category value effect on standard C functions/macros LC_CTYPE LC_TIME LC_NUMERIC LC_MONETARY LC_ALL ? ? ? ? ? Affects behavior of the macros Affects date and time format. Affects number representation format Affects monetary values format combines the affect of all above Permit function pointers without dereferencing ANSI C specifies that a function pointer may be used like a function name.No referencing is needed when calling a function whose address is contained in the pointer. For Example, the following statement given below d efines a function pointer funptr, which contains the address of the function foo. extern void foo(double xyz,const int *ptr); void (*funptr)(double,const int *)=foo; The function foo may be invoked by either directly calling foo or via the funptr. foo(12. 78,†Hello world†); funptr(12. 78,†Hello world†); K&R C requires funptr be dereferenced to call foo. (* funptr) (13. 48,†Hello usp†); ANSI C also defines a set of C processor(cpp) symbols, which may be used in user programs.These symbols are assigned actual values at compilation time. cpp SYMBOL USE _STDC_ Feature test macro. Value is 1 if a compiler is ANSI C, 0 otherwise _LINE_ Evaluated to the physical line number of a source file. _FILE_ Value is the file name of a module that contains this symbol. _DATE_ Value is the date that a module containing this symbol is compiled. _TIME_ value is the time that a module containing this symbol is compiled. The following test_ansi_c. c program illustrates the use of these symbols: #include int main() { #if _STDC_==0 printf(â€Å"cc is not ANSI C compliant†); #else printf(â€Å"%s compiled at %s:%s.This statement is at line %d †, _FILE_ , _DATE_ , _TIME_ , _LINE_ );

Beneficios migratorios para familiares de militares

Los familiares indocumentados de militares en activo, miembros de la Reserva y veteranos licenciados con honor pueden obtener importantes beneficios migratorios a travà ©s del programa Parole in Place (PIP, por sus siglas en inglà ©s). Beneficios migratorios de Parole in Place (PIP) El Parole in Place permite a migrantes indocumentados que son familiares de militares permanecer en Estados Unidos por periodos prorrogables de un aà ±o. Ademà ¡s, los beneficiarios pueden solicitar un permiso de trabajo, si prueban necesidad de trabajar. Asimismo, mientras el Parole in Place està ¡ vigente, los migrantes beneficiados por este programa se encuentran en estatus migratorio legal al considerà ¡rseles parolees y recibir un documento I-94. Tampoco acumulan dà ­as de presencia ilegal ni  tampoco pueden ser deportados por esta causa. Finalmente, cabe destacar que en los casos en los que el migrante que obtiene el Parole in Place es cà ³nyuge, progenitor o hijo soltero menor de 21 aà ±os de un ciudadano estadounidense podrà ­a solicitar un ajuste de estatus. Mediante este trà ¡mite, obtener la tarjeta de residencia permanente, tambià ©n conocida como green card.  ¿Quià ©nes pueden solicitar Parole in Place? Para solicitar exitosamente el Parole in Place deben cumplirse una serie de requisitos. Và ­nculo familiar El migrante para el que se pide el Parole in Place debe ser esposo/a, padre, madre, viudo/a, hijo/a de un militar alistado en la actualidad en una de las cinco ramas del Ejà ©rcito de Estados Unidos (Marine Corps, Navy, Army, Coast Guard y Air Force). Tambià ©n se admite que se trate un miembro de la Selected Reserve of the Ready Reserve o de un veterano –vivo o fallecido– que fue licenciado con honor del Ejà ©rcito o de la Reserva. Ingreso ilegal a Estados Unidos El ingreso ilegal a Estados Unidos sin autorizacià ³n por parte de un oficial migratorio es una condicià ³n indispensable para que un migrante pueda beneficiarse de este programa. En otras palabras, este programa aplica a los migrantes que ingresaron ilegalmente, pero no aplica para los migrantes indocumentados que ingresaron legalmente con una visa y en la actualidad està ¡n ilegalmente en el paà ­s porque han permanecido mà ¡s tiempo que el permitido. Para los indocumentados que ingresaron legalmente puede haber otras opciones, pero no el Parole in Place. Estatus migratorio del militar El militar, reservista o veterano familiar del migrante indocumentado para el que se solicita el Parole in Place tiene que ser ciudadano estadounidense o residente permanente legal. Decisià ³n de USCIS Para que USCIS apruebe una peticià ³n debe considerar que hay una razà ³n humanitaria que lo justifique o un beneficio pà ºblico significante. Con carà ¡cter general, USCIS ha venido aprobando las peticiones en las que el à ºnico problema migratorio era la presencia ilegal. La peticià ³n puede negarse si hay otros problemas, como condenas por delitos u otras violaciones migratorias. La decisià ³n para aprobar o negar la solicitud depende de USCIS, quien tiene poder discrecional.  ¿Cà ³mo se solicita el Parole in Place y cuà ¡nto cuesta? El formulario de USCIS que debe completarse es el I-131, Aplication for Travel Document. En la Parte 2, en vez de marcar una casilla, debe escribirse a mano Military PIP. La presentacià ³n de esta planilla no tiene costo para el solicitante. Este formulario, junto con la documentacià ³n de apoyo, debe enviarse a la oficina de USCIS que corresponda segà ºn el lugar de residencia habitual. En el caso de familias militares destinadas a un lugar diferente al de su domicilio habitual, tambià ©n pueden presentar la solicitud en la oficina de USCIS que corresponda a su lugar de destino militar. Documentacià ³n de apoyo La solicitud de Parole in Place a travà ©s del formulario I-131 debe acompaà ±arse de una serie de documentos. En primer lugar, fotocopia de documento que pruebe la relacià ³n entre el solicitante del beneficio migratorio y el militar, reservista o veterano. Por ejemplo, el certificado de matrimonio o el de defuncià ³n, en caso de viudos. Si se trata de una relacià ³n filial, el certificado de nacimiento. Tambià ©n es và ¡lido presentar prueba de membresà ­a en Defense Enrollment Eligibility Reporting System (DEERS, por sus siglas en inglà ©s). En segundo lugar, dos fotos del solicitante del beneficio. Deben ser idà ©nticas y tipo pasaporte. En tercer lugar, prueba del estatus militar, reservista o veterano. Por ejemplo, el I.D. militar. Y, en cuatro lugar, cualquier documento que sirva para probar razones humanitarias o de beneficio pà ºblico o cualquier otra razà ³n que se considere puede ser un factor positivo en la peticià ³n. Puede alegarse enfermedad, excelente ajuste en la comunidad, etc. Si algà ºn documento està ¡ en un idioma distinto al inglà ©s, debe traducirse y certificarse siguiendo un modelo de carta.  ¿Cuà ¡nto tiempo se demora en obtener el Parole in Place (PIP)? El USCIS no publica cuà ¡nto se demora en aprobar o negar la solicitud de Parole in Place. Sin embargo, se estima que la mayorà ­a de los casos se han venido resolviendo en tres meses o menos. Durante ese tiempo, el solicitante recibirà ¡ una cita para la toma de huellas digitales y otros datos biomà ©tricos. Otros beneficios migratorios para familiares de militares El beneficio de la Deferred Action para familiares de militares aplica a migrantes que no califican ni para un ajuste de estatus ni para el programa Parole in Place. La Deferred Action permite a un migrante con orden de deportacià ³n permanecer por un tiempo en Estados Unidos. Si solicitud es aprobada, el beneficiario podrà ­a solicitar un permiso de trabajo, argumentando necesidad econà ³mica.  ¿Dà ³nde encontrar ayuda legal para el Parole in Place? Para verificar si se califica para solicitar puede contratarse los servicios de un abogado de AILA o solicitar asesorà ­a de organizaciones de apoyo a migrantes con excelente reputacià ³n y con experiencia en este tipo de casos. Ademà ¡s, el ciudadano o residente militar, reservista o veterano puede cerrar una cita con la oficina local de USCIS y pedir hablar con el oficial a cargo de asuntos militares. Asimismo, se puede marcar a la là ­nea de Military Help 877-CIS-4MIL (877-247-4645) Tips No se puede ingresar a ninguna base militar utilizando documentacià ³n tipo matrà ­cula consular. A partir del 1 de octubre de 2020, tampoco se podrà ¡n utilizar licencias de manejar ordinarias y serà ¡ necesario mostrar un documento que cumpla con los requisitos de Real I.D. El programa de Parole in Place està ¡ siendo cuestionado por el gobierno del presidente Donald Trump. Por el momento sigue en activo y se puede solicitar este beneficio. Puntos clave: Parole in Place Este beneficio migratorio es para migrantes indocumentados que ingresaron ilegalmente a Estados Unidos.Deben ser cà ³nyuges, padres, madres o hijos de ciudadanos o residentes permanentes que està ¡n alistados en el Ejà ©rcito, la Reserva o son veteranos licenciados con honor.Los migrantes con este beneficio no pueden ser deportados y pueden sacar permiso de trabajo.Los familiares inmediatos de ciudadanos pueden, despuà ©s de obtener el Parole in Place, obtener la tarjeta de residencia solicitando un ajuste de estatus. Este artà ­culo es meramente informativo. No es asesorà ­a legal para ningà ºn caso concreto.